Sequencing a Microbial Genome
نویسندگان
چکیده
Although many genomes are available for download today, the underlying technologies should not be taken for granted. By using shotgun sequencing techniques and a gauntlet of informatics, we are able to produce high-quality DNA sequence. We will first look at some of the robotics and chemistries of preparing DNA as samples for the sequencing instruments. Then we will look at the series of applications used in taking raw data signals, converting them to sequence and then finally assembling the data into a single genome. Highlighted will be some of the techniques used to speed the informatics processes as well as some of the challenges that informatics faces in processing data and assembling the genome.
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